Anti-PHAP(NT)Antibody

ORDERINGINFORMATION

CatalogNo.:11086

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

SPECIFICATIONSUMMARY

Antigen:SyntheticpeptidecorrespondingtoaminoacidsneartheaminoterminusofhumanPHAP(accessionno.P39687).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide

BACKGROUND

ThePHAPproteins(tumorsuppressorputativeHLA-DRassociatedproteins)areimportantregulatorsofmitochondrialapoptosis.PHAPfacilitatesapoptosome-mediatedcaspase-9activationtostimulatethemitochondrialapoptosispathway.Inaddition,PHAPopposesbothRas-andmyc-mediatedcelltransformation.

SPECIFICITY

Thisantibodyrecognizesallthreeisoformsofhuman,mouseandratPHAP,includingPHAPI(32kDa),PHAPI2a,andPHAPIII(35kDa).

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

APPLICATIONS

Immunoblotting:Useat1ug/ml.

WesternblotanalysisofPHAPinRajicelllysatewithPHAPantibodyat1μg/ml.ThewideandstrongbandbelowPHAPIIIisadoubletcomposedofPHAPI(upper)andPHAPI2a(lower).

Positivecontrol:Rajicelllysate.

Immunocytochemistry:useat1ug/ml.

ImmunocytochemicalstainingofPHAPinRajicellswithPHAPantibodyat1μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

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