Anti-Caspase-13Antibody

ORDERINGINFORMATION

CatalogNo.:2245

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

BACKGROUND

Apoptosisisrelatedtomanydiseasesandinducedbyafamilyofcelldeathreceptorsandtheirligands.Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.Caspase-13orERICE(EvolutionarilyRelatedInterleukin-1ConvertingEnzyme)wasrecentlydescribed.Whenactivatedbycaspase-8,caspase-13inducesapoptosis.Caspase-13isexpressedinavarietyofhumantissuesandcelllines.

SPECIFICATIONSUMMARY

Antigen:Peptidecorrespondingtoaa91-105ofhumancaspase-13(accessionno.O75601).

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

SPECIFICITY

Thisantibodyrecognizeshuman,mouse,andratcaspase-13(43kDa).

APPLICATIONS

Immunoblotting:useat1ug/ml.

WesternblotanalysisofCaspase-13in(A)HL60celllysate,(B)mousebrainand(C)ratbraintissuelysateswithCaspase-13antibodyat1μg/ml.

Immunohistochemistry:useat10ug/ml.

ImmunohistochemicalstainingofCaspase-13inmousebraintissuewithCaspase-13antibodyat10μg/ml.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:WholecelllysatefromHL60cells.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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