productdatasheet
CatalogNo.:45102
Size:20ug
Source:Genetically-engineeredE.coli.MolecularWeight:~17,000daltonsPurity:>90%
Diluent:PBS/1.5MNaCl
Preservatives:None
Purifiedbyheparinaffinitychroma-tography.
TheactivityofratFGF-7at1-10ng/mlisdeterminedbytritiatedthymidineincorporationusingculturedmousekeratinocytes.Maximumstimulationobservedat1ng/ml.Theoptimalconcentrationofthisproductfor
usewithothercelltypesmustbedeterminedbytheenduser.
SPECIFICITY45102bindstokeratinocytesandmostparenchymalepithelialcells;bindsspecificsplicevariantofFGFreceptorexpessedinepithelialcells(FGFr2IIIb).
Dilutewitheither0.1%TFA/40%acetonitrileorPBS/0.1%CHAPSforbeststABIlityandtopreventabsorptionofproductonwallsofvessels.
Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Keepfrozenuntiluse.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
Yan,G.(1991)InVitroCellDevBiol27A:437;Wang,F.etal(1995)JBiolChem270:10222.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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