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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Cataloguenumber
PRO-1423
Synonyms
F52,MACMARCKS,MLP,MLP1,MRP,MARCKS-relatedprotein,MARCKS-likeprotein1,Macrophagemyristoylatedalanine-richCkinasesubstrate,MARCKSL1.
Introduction
MARCKS-relatedprotein(MARCKSL1)whichisapartofMARCKSfamilyofPKCsubstrateisbroadlyusedinthesignaltransductionstudiesasanindicatorofPKCactivation.MARCKSL1playsapartinthecoordinationofmembrane-cytoskeletalsignalingevents,includingsecretion,migration,phagocytosisandcelladhesionandisexpressedinavarietyoftissueswithhighestlevelsfoundintestisanduterus.FurThermore,MARCKSL1servesasaregulatorofIntegrinactivationandisthoughttoregulateIntegrin-dependentsignaltransductionpathways,particularlythoseinvolvedinmacrophagespreADIng.
Description
MARCKSL1HumanRecombinantproducedinE.coliisasinglepolypeptidechaincontaining218aminoacids(1-195)andhavingamolecularmassof21.9kDa.MARCKSL1isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheMARCKSL1solution(0.25mg/ml)contains20mMTris-HClbuffer(pH8.0),0.1MNaCland20%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMGSQSSKAPRGDVTAEEAAGASPAKANGQENGHVKSNGDLSPKGEGESPPVNGTDEAAGATGDAIEPAPPSQGAEAKGEVPPKETPKKKKKFSFKKPFKLSGLSFKRNRKEGGGDSSASSPTEEEQEQGEIGACSDEGTAQEGKAAATPESQEPQAKGAEASAASEEEAGPQATEPSTPSGPESGPTPASAEQNE.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.