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商品描述
Cataloguenumber
PRO-1347
Synonyms
KeratintypeIcytoskeletal19,Cytokeratin-19,CK-19,Keratin-19,K19,KRT19,CK19,K1CS,MGC15366.
Introduction
CTK-19isamemberofthekeratinfamily.ThekeratinsareintermediatefilamentproteinsresponsIBLeforthestructuralintegrityofepithelialcellsandaresuBDividedintocytokeratinsandhairkeratins.ThetypeIcytokeratinsconsistofacidicproteinswhicharearrangedinpairsofheterotypickeratinchains.Unlikeitsrelatedfamilymembers,thissmallestknownacidiccytokeratinisnotpairedwithabasiccytokeratininepithelialcells.Itisspecificallyexpressedintheperiderm,thetransientlysuperficiallayerthatenvelopesthedevelopingepidermis.ThetypeIcytokeratinsareclusteredinaregionofchromosome17q12-q21.
Description
KRT19HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining423aminoacids(1-400)andhavingamolecularmassof46.5kDa.
KRT19isfusedtoa23aminoacidHis-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.
KRT19isfusedtoa23aminoacidHis-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheKRT19solution(0.5mg/ml)contains20mMTris-HClbuffer(pH8.0),0.4MUreaand10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleaseavoidfreezethawcycles.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleaseavoidfreezethawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMTSYSYRQSSATSSFGGLGGGSVRFGPGVAFRAPSIHGGSGGRGVSVSSARFVSSSSSGAYGGGYGGVLTASDGLLAGNEKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.


