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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Cataloguenumber
ENZ-249
Synonyms
MGC2652,THTP,THTPASE.
Introduction
THTPAenzymeispartoftheTHTPasefamily.THTPAislocalizedtothecytoplasmandexpressedatsmallquantitiesinavarietyoftissues,includingtestis,uterus,prostate,bladder,lungandkidney.THTPAisahydrolasethatcatalyzestheH2O-dependenthydrolysisofthiaminetriphosphate(THTP)tothiaminediphosphate(THDP),themainformofthiaminewithinthecell.THTPAoccursasamonomerandisactivatedatanoptimalpHof8.5.
Description
RecombinantHumanTHTPAproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining250aminoacids(1-230a.a.)andhavingamolecularmassof27.7kDa.THTPAisfusedtoa20aminoacidHis-TagatN-Terminusandpurifiedbyconventionalchromatographytechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
TheTHTPA1mg/mlproteinsolutioncontains20mMTris-HCLbuffer,pH-8,1mMDTTand10%Glycerol.
StABIlity
StoreTHTPAat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMAQGLIEVERKFLPGPGTEERLQELGGTLEYRVTFRDTYYDTPELSLMQADHWLRRREDSGWELKCPGAAGVLGPHTEYKELTAEPTIVAQLCKVLRADGLGAGDVAAVLGPLGLQEVASFVTKRSAWKLVLLGADEEEPQLRVDLDTADFGYAVGEVEALVHEEAEVPTALEKIHRLSSMLGVPAQETAPAKLIVYLQRFRPQDYQRLLEVNSSRERPQETEDPDHCLG.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.