Cataloguenumber
PRO-2493
Synonyms
Lymphocytefunction-associatedantigen3isoform1,CD58,ag3,LFA-3,LFA3,SurfaceglycoproteinLFA-3.
Introduction
CD33isputativeadhesionmoleculeofmyelomonocytic-derivedcellswhichmediatessialic-aciddependentbindingtocells.CD33preferstobindtoalpha-2,6-linkedsialicacid.Thesialicacidrecognitionsiteismaskedbycisinteractionswithsialicacidsonthesamecellsurface.Intheimmuneresponse,CD33performasaninhibitoryreceptoruponligandinducedtyrosinephosphorylationbyrecruitingcytoplasmicphosphatasesthroughtheirSH2domainswhichblocksignaltransductionthroughdephosphorylationofsignalingmolecules.Inaddition,CD33inducesapoptosisinacutemyeloidleukemia.
Description
CD58producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining196aminoacids(29-215a.a.)andhavingamolecularmassof22.5kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).CD58isexpressedwitha9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
CD58proteinsolution(1mg/ml)containsphosphatebufferedsaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
SafetyDataSheet
SDS
Aminoacidsequence
ADPFSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEHYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHRHHHHHH