Catalogue number
CYT-295
Synonyms
Somatomedin C, IGF-I, IGFI.
Introduction
The somatomedins, or IGFs, comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of GH. Early studies showed thatGH did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed "sulfation factor," which later became known as somatomedin.Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2), and somatomedin B.
Description
IGF1 Gilthead SeabreamRecombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7545.4 Dalton, the predicted pI=7.72.IGF-1 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Solubility
It is recommended to reconstitute the lyophilized IGF-1 in sterile 0.4% NaHCO3 adjusted to ph 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
LyophilizedIGF1although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity
Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
Amino acid sequence
MSPETLCGAELVDTLQFVCGERGFYFSKPGYGPNARRSRGIVDECCFQSCELRRLEMYCAPAKTSK
Biological Activity
Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active.
Protein content
Somatomedin C quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.60 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.
Safety Data Sheet
Usage
ProSpec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec是全球著名细胞因子蛋白及相关抗体生产和研发品牌，专注于蛋白生产研发，独有细菌和哺乳动物表达和蛋白折叠技术。目前可提供细胞因子、生长因子、激素、信号蛋白、病毒抗原等近800种重组蛋白和100多种抗体。
ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.
Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....
产品列表：
No.
品牌
货号
名称
规格
1
ProSpec
PRO-1574
BCL2 Human
5µg
2
ProSpec
CYT-1075
IFNA7 Human, Sf9
2µg
3
ProSpec
CHM-328
Rantes Human
5µg
4
ProSpec
CHM-330
IP 10 Human
5µg
5
ProSpec
CYT-575
MIF Human
5µg
6
ProSpec
CYT-538
GH Antagonist Chicken
20µg
7
ProSpec
CYT-1043
TNFRSF10A Human
2µg
8
ProSpec
PRO-2390
CD2 Human, Sf9
2µg
9
ProSpec
PRO-2237
CD47 Human
2µg
10
ProSpec
CYT-581
Beta NGF Mouse
5µg
11
ProSpec
CYT-973
NRP1 Rat
1µg
12
ProSpec
HOR-253
FSH Human
2µg
13
ProSpec
HOR-275
Thymosin beta 4
1mg
14
ProSpec
DEN-005
Dengue Envelope-1 22kDa
100µg
15
ProSpec
HEV-235
HEV Mosaic
100µg
