Type:CellGrowthMedia
Applications:AdherentCellAssays
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysFormat:liquid-liquid
RatNeurosphere/ProgenitorDifferentiationMedia isaserumfreemediumcontainingNeurobasal®,B27®(withoutretinylacetate),Glutamax™,FGF2,andEGFthathasbeenoptimizedforthegrowthandproliferationofneurospheresinculture(PM:17545985).Derivedfromneuralstemcells,neurospheresarefree-floatingclustersofneuroprogenitorcellswiththeABIlitytodifferentiateintoneurons,astrocytesandoligodendrocytes. TheMedia isanexcellentchoiceforscale-upofneuroprogenitorcellsinhighthroughputapplications.
Mediashouldbestoredat4°Cforuptotheexpirationdatelistedonthelabel.Itshouldnotbefrozen.Thismediumisdesignedforuseat37°Cina5%CO2incubator.Neurospheresaresubjecttolessoxidativestressiftheoxygenisalsoregulatedat9%.
Image:DifferentiatingRatCorticalStemCells.NeuralprogenitorswerelabeledwithNestin antibody.Differentiatedneuronswerelabeledwithneuron-specific Tuj1(Neuron-specificclassIIIbeta-tubulin) antibody.
