Type:Protein
Applications:WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:liquid-liquid
Immunogen:RecombinantartificialmosaicproteincontainstheCMVgBimmunodominantregions,60-81aaofgBproteinstrainAD169repeatedthreetimes.
CMVbelongstotheBetaherpesvirinaesubfamilyofHerpesviridaewhichincludesherpessimplexvirustypes1and2,varicella-zostervirus,andEpstein-Barrvirus.TheherpesvirusesshareacharacteristicABIlitytoremainlatentoverlongperiods.CMVisadouble-strandedlinearDNAviruswith162hexagonalproteincapsomeressurroundedbyalipidmembrane.CMVhasthelargestgenomeoftheherpesviruses,rangingfrom230-240kilobasepairs.
HumanCMViscomposedofuniqueandinvertedrepeatsthatincludetheexistenceof4genomeisomerscausedbyinversionofL-Sgenomecomponents(classE).Replicationmaybedividedintoimmediateearly,delayedearly,andlategeneexpressionbasedontimeofsynthesisafterinfection.TheDNAisreplicatedbyrollingcircles.Invitro,CMVreplicatesinhumanfibroblasts.
Specificity:ImmunoreactivewithseraofCMV-infectedindividuals.
Applications:CMVMosaicantigenissuitableforELISAandWesternblots,excellentantigenfordetectionofCMVwithminimalspecificityproblems.
OtherReagentstoConsider:
CytomegaloVirusPp28(UL99)
