Type:Protein
SpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All
Neurofilamentsarethe10nmorintermediatefilamentproteinsfoundspecificallyinneurons,andarecomposedpredominantlyoffourmajorproteinscalledNF-L,NF-M,NF-Handα-internexin.NF-ListheneurofilamentlightorlowmolecularweightpolypeptideandrunsonSDS-PAGEgelsatabout68kDa.
ACDNAencodingfulllengthhumanneurofilamentNF-LwasinsertedintoaneukaryoticexpressionvectorwhichaddsanN-terminalinframeHis-tag.ThiswastransformedintoE.coliandrecombinantproteinwaspurifiedin6Mureausingimmobilizedmetalaffinitychromatography.Purifiedproteinisdilutedto1mg/mLandissuppliedin6Murea.
Image:CoomassieBrilliantBluestainedSDS-PAGEgelofvariousrecombinantproteins.His-taggedhumanNeurofilamentNF-L,wasexpressedandpurifiedfromE.coliBL21usingimmobilizedmetalaffinitychromatography.1µgofproteinwasrunoneachlane,andthelaneindicatedwith"NF-L"containstheNeurofilamentNF-Lprotein.TheotherlanesshowrecombinantHis-taggedPeripherin,α-InternexinandVimentinasindicated.Proteinmolecularweightstandardsareinthefirstlaneandapparentmolecularweightsareasindicated.
