Neuromics/Cyckin-C (CCNC)/PR27045-2/2 ug

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¥2160.00
货号:PR27045-2
浏览量:127
品牌:Neuromics
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商品描述

Type:Protein

 

SpeciesReactivity:H

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

Picture
rCCNC(cyclinC)isamemberofthecyclinfamilyofproteins.Thisproteininteractswithcyclin-dependentkinase8(CDK8)andinducesthephophorylationofthecarboxy-terminaldomainofthelargesubunitofRNApolymeraseII.ThelevelofmRNAsforthisproteinpeaksintheG1phaseofthecellcycle.Ithasbeenshownthatanon-cdk8-associatedcellularpoolofCCNCcombineswithcdk3tostimulatepRbphosphorylationatS807/811duringtheG0/G1transition,andthatthisphosphorylationisrequiredforcellstoexitGOefficiently.OtherresultshaveshownthatamarkedelevatedimmunoreactivityofCCNCwasfoundbothinneuronsandastrocytesinAlzheimer"sdisease(AD).IncreasedlevelsofCCNCRNAweredetectedbyribonucleaseprotectionassay(RPA)insevereADcasesandcolocalizationofCCNCanditspreferredbindingpartner,CDK8,wasonlyobservedinastrocytesbutnotinneurons.
RecombinantCNCCisexpressedinE.coliasafull-lengthproteinfusedtoaproprietary16-Kdtag(a6xHistagislocatedattheveryN-terminalend).

Image:CNCCStructureandSequence.

Applications:

RecombinanthumanCNCCcanbeuseddirectlyasapositivecontrolinWesternblotting,ELISA,immunoprecipitationandotherimmunologicalexperiments.TheBIOLOGicalactivityofthisproducthasnotyetbeentested.

References:

1.GeneExpressionsSpecificallyDetectedinMotorNeurons(Dynactin1,EarlyGrowthResponse3,Acetyl-CoATransporter,DeathReceptor5,andCyclinC)DifferentiallyCorrelatetoPathologicMarkersinSporADIcAmyotrophicLateralSclerosis. JNeuropatholExpNeurol2007Jul;66(7):617-627.
2.TwoisoformsofthehumancyclinCgeneareexpresseddifferentiallysuggestingthattheymayhavedistinctfunctions. ActaBiolHung2007Mar;58(1):133-7.
3.OsmosensitivegeneexpressionoftaurinetransporterandcyclinCinembryonicfibroblastcells. AdvExpMedBiol2006;583:49-57.
4.Highlyfrequentalleliclossofchromosome6q16-23inosteosarcoma:involvementofcyclinCinosteosarcoma. IntJMolMed2006Dec;18(6):1153-8.
5.RegulationoftheoxidativestressresponsethroughSlt2p-dependentdestructionofcyclinCinSaccharomycescerevisiae. Genetics2006Mar;172(3):1477-86.
6.[InfluenceofaerosolsontheexpressionofcyclinB1,cyclinCandproliferatingcellnuclearantigeninwoundtissuehealingofburnedratmodels] ZhonghuaWaiKeZaZhi2005Oct1;43(19):1280-3. 

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