Type:GuineaPigIgG
Applications:ICC;IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-liquid
Immunogen:(C)TASLDPDDFDPEPSDPLGSP
Theacid-sensingionchannelfamily(ASIC)comprisessixdiscreteASICsubunits(ASIC1A,ASIC1B,ASIC2A,ASIC2B,ASIC3,ASIC4)thatdetecttissueacidosis(i.e.adecreaseinpH).Thisacidosisphenomenon isa responsetotissueinjury,pain andinflammation.
Figure:ASIC3expressioncomparedtoothernociceptiveionchannels.DRGsectionsdouble-labeledforASIC3(red)andeitherP2X3(green,A)orTRPV1(green,B);double-labeledcellsareyellowororange.CirclechartsshowtherelativedistributionofthethreereceptorsamongallDRGneurons.ASIC3andP2X3expressionoverlappedinonly4%ofneurons,whilesome35%ofASIC3-positiveneuronsalsoexpressTRPV1.TherarecellspositiveforbothASIC3andP2X3werelarge(eg.cellatlowerleftinA).1406cellscountedforA;995forB.C,D,electrophysiologicalrecordingsrevealaqualitativelysimilarexpressionpatternforcurrentsconformingtoASIC3,P2X3,orTRPV1.ThecellthatgeneratedthecurrentsinCcountsasaco-expressorofASIC3andP2X3,fittingintotheyellowbininthecircleplot.ThecellinDisaco-expressorofASIC3andTRPV1.Cellswerecountedpositiveiftheyhadatleast0.3nAofcurrentinresponsetopH6.8(ASIC3+),50μMATP(P2X3+;transientcurrentonly),or1μMcapsaicin(TRPV1).182cellsrecordedforC;169forD.
ASIC3:CustomerPublications
