Type:RabbitIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;Pr;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-liquid
Immunogen:RecombinantfulllengthhumanPGP9.5orUCHL1purifiedfromE.coli.
PGP9.5,Park5 orUCHL1 isasolublecytoplasmicproteinwithamolecularweightofapproximately25,000kD.Itispresentinneuronsandincellsofthediffuseneuroendocrinesystem.ItisanexcellentMarkerforneuronsoftheCNSandPNS.
Image:Mixedratneuron/glialculturesstainedwithPGP9.5/UCHL1(green).BlueisaDNAstain.NotethattheUCHL1stainsneuronsstronglyandspecifically,andthatthestainingisconcentratedinthecellbodies,thoughsomedoesextendintothedendritesalso.Surroundingglialcellsarenotstainedwiththisantibody,thoughmanyarepresentasvisualizedusingtheDNAstain.Inset:BlotofwholebovinebrainextractstainedwithPGP9.5/UCHL1showingastrongandcleanbandatabout24kDa.ProtocolonData-sheet.
Thegreatabundanceofthisproteininneuronsmeansthatitisreleasedfromneuronsinlargeamountsfollowinginjuryordegenerationand isdetected inCSFandotherbodilyfluids.Itis usedasa bio-markerforParkinson"sDisease(PD),Alzheimer"sDisease(AD),Axonaldystrophiesand TraumaticBrainInjury(TBI).
