Type:MouseIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AscitesFluid-liquid
Immunogen:RecombinantfulllengthhumanUCHL1purifiedfromE.coli.
UbiquitinC-terminalhydrolase1(UCHL1)hasseveralothernames,suchasubiquitincarboxylesteraseL1,ubiquitinthiolesterase,neuron-specificproteinPGP9.5andPark5.Theproteinisextremelyabundant,andwasestimatedtobepresentataconcentrationof200-500micrograms/gwetweight,representingamajorproteincomponentofneuronalcytoplasm(1).Thishasbeenclaimedtorepresent1-2%oftotalbrainprotein.
Image:ShowsasectionofratspinalcordstainedwithUCHL1(red)andneurofilamentNF-Hantibody(green).Thelargecellsarea-motorneuronsandUCHL1fillsthecytoplasmoftheirperikaryaanddendrites.TheneurofilamentNF-HantibodybindsprimarilytophosphorylatedaxonalformsofNF-H,andsostainsaxonscoursingbetweenthelargemotorneurons.Protocolondata-sheet.
Theactivityisalsoimportanttoremoveubiquitinfrompartiallydegradedproteins,allowingtheubiquitinmonomertoberecycled.Regulationoftheubiquitinpathwayisveryimportantandmanydiseasestatesareassociatedwithdefectsinthispathway.PointmutationsintheUCHL1geneareassociatedwithsomeformsofhumanParkinson"sdisease.RecentstudiessuggestthatUCHL1alsohasaubiqutinylligaseactivity,beingabletocoupleubiquitinmonomersbylinkingtheC-terminusofonewithlysine63oftheother.SinceUCHL1isheavilyexpressedinneurons,antibodiestoUCHL1canbeusedtoidentifyneuronsinhistologicalsectionsandintissueculture.Thegreatabundanceofthisproteininneuronsmeansthatitisreleasedfromneuronsinlargeamountsfollowinginjuryordegeneration,sothedetectionofofUCHL1inCSFandotherbodilyfluidscanbeusedasabioMarker.UCHL1wasalsodiscoveredasagenemutatedinsomerarefamialformsofParkinson"sdisease.InterestinglyacommonallelicvariantofUCHL1,theS18YpolymorphismisactuallyprotectiveagainstParkinson"sdisease. Itisalsoamarkerfor cellsofthediffuseneuroendocrinesystemandtheirtumors.
