Type:MouseIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;P;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AscitesFluid-liquid
Immunogen:FulllengthrecombinanthumanTDP43expressedinandpurifiedfromE.coli.
TarDNABindingProtein,TDP43orTARDBPwasoriginallyidentifiedasatranscriptionalrepressorthatbindstochromosomallyintegratedTARDNAandrepressesHIV-1transcription.ItwasalsoreportedtoregulatealternatesplicingoftheCFTRgeneandtheapoA-IIgene.
Image:TDP43stainingofformalinfixedadultratbrain,specificallythehippocampus.Hippocampalneuronnucleiarestainedstrongly.ChickenantibodytoGFAP(green)showstheprocessesofastrocyticglialcells.NucleiofallcellsarerevealedwithDAPIDNAstain(blue).TheTARDPantibodystainsneuronalnucleistronglyandthenucleiofsomenon-neuronalcellsmuchmoreweakly.Neuronalnucleithereforelookcrimson,sincetheyarebothredduetothecontentofTDP43andblueduetotheircontentofDNA,stainedbluewithDAPI.Protocolondata-sheet.
Lateritwasdiscoveredthathyper-phosphorylated,ubiquitinatedandcleavedformofTARDBP,knownaspathologicTDP43,isthemajordiseaseproteininubiquitin-positive,tau-,andalpha-synuclein-negativefrontotemporaldementia(FTLD-U)andinAmyotrophiclateralsclerosis(ALS).ElevatedlevelsoftheTDP-43proteinhavealsobeenidentifiedinindividualsdiagnosedwithchronictraumaticencephalopathy,aconditionthatoftenmimicsALSandthathasbeenassociatedwithathleteswhohaveexperiencedmultipleconcussionsandothertypesofheadinjury.
