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蚂蚁淘在线 / 品牌中心 / Neuromics / Neuromics/IL-8 Sandwich ELISA Kit/ELH-IL8-003/5 Plate Kit

Neuromics/IL-8 Sandwich ELISA Kit/ELH-IL8-003/5 Plate Kit

价格
￥28980.00
货号：ELH-IL8-003
浏览量：127
品牌：Neuromics
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商品描述

Type:Kit

 

Applications:E

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

SpeciesReactivity:H

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

EL-8 Sandwich ELISA Kit level in Smoker

Sensitive,SpecificandCostEffective

Interleukin-8(IL-8)isamemberofafamilyofstructurally-relatedlowmolecularweightproinflammatoryfactorsknownaschemokines.IL-8isproducedbystimulatedmonocytesbutnotbytissuemacrophagesandTlymphocytes.IL-8isanon-glycosylatedproteinof8kDa(72aminoacids).Itisproducedbyprocessingofaprecursorproteinof99aminoacids

IL-8induceschemotaxisintargetcells,primarilyneutrophilsbutalsoothergranulocytes,causingthemtomigratetowardthesiteofinfection.IL-8alsoinducesphagocytosisoncetheyhavearrived.IL-8isalsoknowntobeapotentpromoterofangiogenesis

Figure:Figure1.SerumlevelofIL-8.TheresultsaremeanandSEM(n=34).#Statisticallysignificant(Studentt-test,p<0.001).doi:10.4236/ojcd.2013.31002PublishedOnlineMarch2013

SuggestedApplication:QuantitativeProteinDetection;EstablishingNormalRange;ValidationofAntibodyArrayResults.

KitComponents:
·  Pre-Coated96-WellStripMicroplate
·  WashBuffer
·  StopSolution
·  AssayDiluent(s)
·  LyophilizedStandard
·  BiotinylatedDetectionAntibody
·  Streptavidin-ConjugatedHRP
·  TMBOne-StepSubstrate
OtherMaterialsRequired:
·    Distilledordeionizedwater
·    PrecisionPipettestodeliver2μlto1mlvolumes
·    Adjustable1-25mlpipettesforreagentpreparation
·    100mland1litergraduatedcylinders
·    Tubestopreparestandardandsampledilutions
·    Absorbentpaper
·    Microplatereadercapableofmeasuringabsorbanceat450nm
·    Log-loggraphpaperorcomputerandsoftwareforELISAdataanalysis
ProtocolOutline:
1.Prepareallreagents,samplesandstandardsasinstructedinthemanual.
2.Add100μlofstandardorsampletoeachwell.Incubate2.5hatRTorO/Nat4°C.
3.Add100μlofpreparedbiotinantibodytoeachwell.Incubate1hatRT.
4.Add100μlofpreparedStreptavidinsolutiontoeachwell.Incubate45minatRT.
5.Add100μlofTMBOne-StepSubstrateReagenttoeachwell.Incubate30minatRT.
6.Add50μlofStopSolutiontoeachwell.Readat450nmimmediately.

 

Figure:BoxplotoftheserumIL-6levelintheRApatientsandcontrolaccordingtotheIL-6(−174G/C)promoterpolymorphismtype.http://dx.doi.org/10.1016/j.ejr.2012.11.002

 

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