Type:RabbitIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-lyophilized
Immunogen:Purified,E.coli-derivedrecombinanthumanp38alphamitogen-activatedproteinkinase(MAPK14)alpha(GenBankaccession#Q16539).
Thep38Mitogen-activatedProteinKinases(MAPKs)areafamilyoffourrelatedSer/Thrkinasesactivatedbyproinflammatorycytokinesandenvironmentalstresses.
Themostfrequentlyanalyzedfamilymember,p38alpha,alsoknownasSAPK2aandMAPK14,wasinitiallypurifiedasakinasecriticaltothesignalingcascadelinkingIL-1toMAPKAPK-2andthesmallheatshockproteinHSP27.Ubiquitouslyexpressed,p38alphaisduallyphosphorylatedbyMKK3andMKK6atThr180andTyr182.Onceactivated,p38alphaphosphorylatesanumberoftargets,includingthecytoplasmickinasesMNK4andPRAK5andthenucleartranscriptionfactorsATF21andSTAT1.Severalpromisingcompoundsthatinhibitp38alphaarebeinginvestigatedaspotentialtherapiesforarthriticandinflammatorydiseases.
Image:OrexinBandp38alpha weredetectedinfrozensectionsofmousebrainstemusingmouseanti-humanOrexinBmonoclonalantibody(red)andrabbitanti-human/mouse/ratp38alphaaffinity-purifiedpolyclonalantibody(green).
