Type:GoatIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Recombinanthumanextracellularsignal-regulatedkinase-2
ERK1andERK2(alsoknownasMAPK3andMAPK1)are44-and42-kDaSer/Thrkinases,respectively.TheyarepartoftheRas-Raf-ERKsignaltransductioncascadeoftenfounddownstreamofgrowthfactorreceptoractivation.ERK1andERK2wereinitiallyisolatedandclonedaskinasesactivatedinresponsetoinsulinandNGF.Theyareexpressedinmost,ifnotall,mammaliantissues.DualthreonineandtyrosinephosphorylationactivatebothERKs,atThr202/Tyr204forhumanERK1andThr185/Tyr187forhumanERK2.
ERK2isinvolvedinvariouscellularproliferation,differentiation,andcellcycleprogressioninresponsetoavarietyofextracellularsignalsmakingitusefulforthestudyofprocesseslikeangiogenesis,neurogenesisandtumOrigenesis.TheactivationofERKbyproinflammatorystimulimayplayimportantrolesininnateimmuneresponsesandautoimmunity.
Image:DetectionofERK2withGT15111. Immunoblot oflysatesfromhumanHeLaandU937cellsandmouseTS1andNIH/3T3cellsusinganti-ERK2antibody.
