Type:MouseIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,insectcelllineSf21-derived,recombinanthumaninsulin-likegrowthfactorIreceptor(rhIGF-IR)extracellulardomain.
IGF-Ireceptorisadisulfide-linkedheterotetramerictransmembraneproteinconsistingoftwoalphaandtwobetasubunits.BoththealphaandbetasubunitsareencodedwithinasinglereceptorprecursorCDNA.Theproreceptorpolypeptideisproteolyticallycleavedanddisulfide-linkedtoyieldthematureheterotetramericreceptor.ThealphasubunitofIGF-Ireceptorisextracellularwhilethebetasubunithasanextracellulardomain,atransmembranedomainandacytoplasmictyrosinekinasedomain.TheIGF-Ireceptorishighlyexpressedinallcelltypesandtissues.
Images:(1)LysateswerepreparedfromNTERA-2humanembryonalcarcinomacells,SK-MEL-28humanmalignantmelanomacells,andG-361humanmalignantmelanomacellsinnon-reducingsamplebuffer.ThelysateswereresolvedbySDS-PAGEandtransferredtoanImmobilon-Pmembrane.Theblotwasdevelopedwith1μg/mLmonoclonalanti-humanIGF-IRantibodyandWesternGloChemiluminescentDetectionSubstrate.(2) IGF-IR(atadilutionof25µg/mL) stainingof paraffin-embeddedhumanskintissue. Tissue stainedwith anti-mouseHRP-DAB(Brown)andcounterstainedwithhaematoxylin(blue).
OtherReagentstoConsider:
Antibodies
IGF-IRFlowCytometry
IGF-II
IGFBP-3
AllNeurotrophinandGrowthFactors
CancerAntibodies
AllDiabetesandObesityAntibodies
Pain/InflammationAntibodies
AllStemCellReagents
Neuron/GlialMarkers
Proteins
NeurotrophinandGrowthFactorProteins
AllDiabetesandObesityProteins
