Type:MouseIgG
Applications:WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AscitesFluid-liquid
Immunogen:HumanBRCA1(residues1314-1864).
BRCA1(breastandovariancancersusceptibilityprotein1)isaRINGfingerproteincontainingaBRCTdomain.BRCA1existsasaheterodimerwith22possIBLeisoforms.Thefulllengthproteinhasareportedmolecularweightof208kD.BRCA1localizestothemitoticspindlemicrotubules,centriolewalls,pericentriolarfibersatcentrosomes.UnphosphorylatedBRCA1localizesonchromosomesfrommetaphasethroughtelophase;phosphorylatedBRCA1residesininnerchromosomalstructure,centrosome,cleavagefurrowduringprophasethroughtelophase,andrelocalizestotheperinuclearregionwhencellsaresubjectedtoIRorUVrADIationinSphase.BRCA1actsasatumorsuppressorandcanfunctionasasecretedgrowthinhibitoryprotein,participateintranscriptioncoupledrepairofoxidativeDNAdamage,X-chromosomeinactivation,andcanfunctionasaE3ubiquitinligase.BRCA1canbetranscriptionallydownregulatedbyEts-2,Brg-1,andHmga-1.BRCA1canbemodifiedbyglycosylation,ubiquitinationandphosphorylationbyCDK4,ATM/ATR,cdk2,andhChk2.TheBRCA1proteinhasbeenreportedtointeractwithRNApolymeraseIIholoenzymeandBARD1.BRCA1containsatleasttwonuclearlocalizationsignalsandisproposedtobeatumorsuppressorprotein.ItisaserinephosphoproteinthatundergoeshyperphosphorylationduringlateG1andSphasesofthecellcycleandistransientlydephosphorylatedearlyafterMphase.BRCA1proteinaltersinaqualitativeandquantitativemannerduringcellcycleprogression.TheamountofBRCA1proteinishighestduringSphaseandremainselevatedtowardG2/M,beforeitdeclinesinearlyG1phase.InheritedlossofBRCA1functionconfersanincreasedsusceptibilityforbothbreastandovariancancer.
Image:BRCA1inMCF-7wholecelllysate.12minuteECLexposure.
OtherReagentstoConsider:
BRCAA1 Antibody
BRCA1Protein
ARC
Nop30
AllCancerAntibodies
