Type:RabbitIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;Pr;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-liquid
Immunogen:FulllengthrecombinanthumanALDH1L1expressedandpurifiedfromE.coli.
![Picture[2]](images/neuromics/2017/A8x9edax66x1.jpg)
ALDH1L1catalyzestheconversionof10-formyltetrahydrofolate,nicotinamideadeninedinucleotidephosphate(NADP+),andwatertotetrahydrofolate,NADPH,andcarbondioxide.ALDH1L1expressionistissue-specificandishighlyexpressedintheliver.
Image:Neuron-glialcellmixtureculturesstainedwithALDH1L1(red)andourmonoclonalantibodyagainstGFAP(green).BlueisaDNAstain.ALDH1L1stainsastrocytesandexcludesfromneuroncells.ALDH1L1stainstheastrocytescellbodyandprocesses,whereasGFAPlabelstheintermediatefilamentoftheCytoskeletoninsubsetofastrocytes.AstrocytesarepositiveforbothALDH1L1andGFAPappearyellow.ALDH1L1alsolabelsmanyastrocytesnotlabeledbyGFAP,whichappearasred.
Inanearlierstudy,Cahoyetal.appliedFACS(Fluorescent-ActivatedCellSorting)toisolateastrocytesfromEGFP(EnhancedGreenFluorescentProtein)transgenic-mouse,thencreatedatranscriptomedatabaseoftheexpressionlevelsof20,000genesbygeneprofilingofneurons,astrocytesandoligodedrocytesusingAffymetrixGeneChipArrays.TheyidentifiedALDH1L1asahighlyandspecificallyexpressedgeneinastrocytes.ALDH1L1ismorewidelyexpressedthroughoutthebrains,whileastrocyteMarkerGFAPshowsmorepredominantexpressioninwhitematter.Inaddition,lossoffunctionorexpressionof ALDH1L1isassociatedwithdecreasedapoptosis,increasedcellmotility,andcancerprogression,suggestingitsroleasabiomarkerandatargetincancertherapy
