Type:GoatIgG
Applications:E;FC;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:E.coli-derived,recombinanthumanCyclooxygenase2(rhCOX2;aa18-604;Accession#P35354).
Cyclooxygenase(COX),alsoknownasprostaglandinG/Hsynthase,isamembraneboundenzymepartlyresponsIBLeforthecatalysisofprostanoidsynthesis.COXisexpressedasatleastthreedifferentisoforms.COX-1isconstitutivelyexpressedandthoughttoregulateanumberof"housekeeping"functionssuchasvascularhemostasis,renalbloodflow,andglomerularfunction.COX-2expressionistightlyregulatedandinducedbyinflammatorymediatorssuchasgrowthfactors,cytokines,andendotoxin.COX-3appearstobemuchmorestrictlyregulatedspatiallyandisobservedingreatestabundanceincerebralcortex.
Image:Cox-2WesternBlots. Lysatesfromhumanperipheralbloodmononuclearcells(PBMCs)andmouseRAW.264cells,untreatedortreatedwithLPSandhumanU937,untreatedortreatedwithPMAandLPSwereresolvedbySDS-PAGE,transferredtoanImmobilon-Pmembrane,andimmunoblottedwith1.0μg/mLgoatanti-COX2
