Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,E.coli-derived,recombinantmousegrowthdifferentiationfactor3(rmGDF-3).
TheTGFpsuperfamilyofligandsplayskeyfunctionsindevelopmentanddisease.Inbothhumanandmouseembryonicstemcells,amemberofthisfamily,GDF3,isspecificallyexpressedinthepluripotentstate.WeshowthatGDF3isaninhibitorofitsownsubfamily,blocksclassicBMPsignalinginmultiplecontexts,interactswithBMPproteinsandisexpressedspecificallyinthenodeduringgastrulationinapatternconsistentwithBMPinhibition.FurThermore,weusegain-andreduction-of-functiontoshowthatinaspecies-specificmanner,GDF3regulatesbothofthetwomajorcharacteristicsofembryonicstemcells:theABIlitytomaintaintheundifferentiatedstateandtheabilitytodifferentiateintothefullspectrumofcell.
GDF-3issUSPectedtobeexpressedincertainformsofcancer.ThesecouldincludeBreastandTesticularCancer.
Image:GDFStainingofmousethymus.Tissuewasstained withgoatHRP-DAB(brown)andcounterstainedwithHematoxylin(blue).
