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商品描述
Type:RatIgG
Applications:E;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:hybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromaratimmunizedwithpurified,E.coli-derived,recombinantmouseSonicHedgehogC-terminalpeptide(rmShhC-terminalpeptide;aa199-437).
Thehedgehog(hh)geneencodingasecretedproteinwasoriginallyidentifiedinDrosophilaasasegmentpolaritygene.ThreevertebratehomologsofhhhavebeenidentifiedincludingSonichedgehog(Shh),Indianhedgehog(Ihh),andDeserthedgehog(Dhh).Theseproteinsareimportantsignalingmoleculesduringembryonicdevelopment.ShhisexpressedinkeyembryonictissuessuchastheHensenÆsnode,thezoneofpolarizingactivityintheposteriorlimbbud,thenotochord,andthefloorplateoftheneuraltube.
