Type:GoatIgG
Applications:IHC;WB;E;NB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,insectcelllineSf21-derived,recombinanthumanhepatocytegrowthfactor(rhHGF).
Hepatocytegrowthfactor(HGF),alsoknownasHepatopoietinA,ismitogenicforavarietyofcelltypes,includingendothelialandepithelialcells,melanocytes,andkeratinocytes.Itisidenticaltoscatterfactor,afibroblast-derivedsolublefactorthatpromotesthedissociationofepithelialandvascularendothelialcellcoloniesinmonolayerculturesbystimulatingcellmigration.HGFissecretedasaninactivesinglechainprecursorandisconvertedtotheheterodimericactiveformbyHGFactivator.
Image:Stainingofparaffin-embeddedtissuesectionsofhumanliver.Tissues werestainedusinganti-goatHRP-DAB(brown)andHaematoxylin(blue)counterstain.