Type:RabbitIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-lyophilized
Immunogen:syntheticpeptidesequencecorrespondingtoaminoacids579-599ofrat5HTtransportercoupledtoKLH.
TheSEROtonintransporter(SERT)isamonoaminetransporter,integralmembraneproteinthattransportstheneurotransmitterserotoninfromsynapticspacesintopresynapticneurons.ThistransportofserotoninbytheSERTproteinterminatestheactionofserotoninandrecyclesitinasodium-dependentmanner.
Thehistochemicalantibodyforserotonin(5HT)transporterisgeneratedinarabbitagainstasyntheticpeptidesequencecorrespondingtoaminoacids579-599ofrat5HTtransportercoupledtoKLH.
Theantibodyproduces4+(maximum)labelingof5HTtransporteratdilutionsof1/200-1/400usingindirectimmunofluorescenceandatdilutionsof1/10,000-1/15,000usingbiotin/streptavidinHRPtechniqueinratraphenuclei,hypothalamus,cortex,andspinalcord.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.ByWesternblotanalysisusingratbrainextractsofcortex,hypothalamus,midbrain,andhindbrain,theantibodyspecificallylabelsasingleband.Immunolabelingiscompletelyabolishedbypre-adsorptionwithsyntheticrat5HTtransporter.
