Type:RabbitIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:syntheticpeptidesequencecorrespondingtoaminoacids17-34oftherat5-HT5Areceptor.
Thehistochemicalantibodyfor5-HT5Areceptorisgeneratedinarabbitagainstsyntheticpeptidesequencecorrespondingtoaminoacids17-34oftherat5-HT5Areceptor.
Theantibodyproduces4+(maximum)labelingof5-HT5Areceptoratdilutionsof1/100-1/300usingbothindirectimmunofluorescenceandbiotin-streptavidin/HRPtechniqueinratcortexandhippocampus.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.Theantibodywascharacterizedbyimmunoblottingandimmunohistochemistry.Immunoblotsofratbrainextractsrevealedthepresenceoftwobandsatmolecularweightsof41and47kD.Thelowerweightbandagreeswiththecalculatedmolecularweightbasedonaminoacidsequence.Thehigherweightmayrepresentglycosylatedreceptorprotein.ImmunohistochemicalstainingofratbraincorrelateswellwithNorthernblotanalysisandinsituhybridizationstudies.Immunolabelingiscompletelyabolishedbypreadsorptionwithsyntheticrat5-HT5Areceptor(17-34).BlastPdatabasesequencehomologysearchesindicatethattheaminoacidsequenceisuniquetoratandmouse5-HT5Areceptor.
