Type:RabbitIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:multipleantigenicpeptideofanN-terminalsyntheticsequencecorrespondingtoaminoacids22-41ofrat5HT2Areceptor
Theaffinitypurifiedhistochemicalantibodyfor5HT2AreceptorwasgeneratedinrabbitagainstamultipleantigenicpeptideofanN-terminalsyntheticsequencecorrespondingtoaminoacids22-41ofrat5HT2Areceptor.
Image:5HT2ARstainingofratcerebralcortex.
Theantibodyproduces4+(maximum)immunolabelingof5HT2Areceptoratdilutionsof1/300-1/500usingbiotin-streptavidin/HRPtechniquewithfree-floatingmethodsonratcortex,amygdalaandhippocampus.Theadditionofintensifyingreagentssuchasnickelammoniumsulfatetothechromogensolutionallowsfurtherdilutiontoapproximately1/1,000ormore.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.Immunolabelingiscompletelyabolishedbypreadsorptionwithsyntheticrat5HT2Areceptor(22-41).ImmunolabelingofWesternblotrevealedasinglebandofapproximatelybetween55and75kDA.ForWesternBlotProtocolSee:DOI:10.1124/jpet.111.18378.
5HT2AR:CustomerPublications
