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商品描述
Type:RabbitIgG
Applications:IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-lyophilized
Immunogen:Wholemolecule5HT
ThehistochemicalantibodyforSEROtoninisgeneratedinarabbitagainstserotonincoupledtobovineserumalbuminwithparaformaldehyde.
Theantibodyproducesa4+indirectimmunofluorescencestainingata1/1,000-1/2,000dilutionanda4+biotin-streptavidin/HRPstainingata1/20,000-1/40,000dilutioninrathypothalamus,raphenucleiandspinalcord.Stainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywith25µgofserotonin/BSA.
CrossreactivityofSerotoninantiserawasexamined.With5µg,10µgand25µgamountsthefollowingsubstancesdidnotreactwithSerotoninantiseradiluted1/20,000usingtheBn-SA/HRPlabelingmethod:5-hydroxytryptophan,5-hydroxyindole-3-aceticacid,anddopamine.
Image;5HTSerotoninstainingofrathypothalamus.
5HT:CustomerPublications
