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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Type:CoatingBuffer
Applications:E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysFormat:liquid
Thisuniqueplatecoatingbufferisforusewithantibodiesandantigens.ThepHandionicstrengthofthisbufferhavebeenspecificallyformulatedtocreateanoptimalcoatingenvironmentwhichmaximizestheadsorptionefficiencyofmostantibodiesandproteinantigensontopolystyreneplatesurfaces.
ThisbufferalsostABIlizescoatedproteinsbymaintainingtheirtertiarythree-dimensionalstructureduringtheadsorptionprocess.Bystabilizingtheadsorbedprotein,antigenicregionsarepreserved,allowingforgreaterbindingreactivitywiththedetectionmolecule,therebyenhancingthespecificsignal.
Bygeneratingahigherspecificsignal,alowerconcentrationofcoatproteinmaybeneededwhenthisbufferisused,therebysavingvaluablereagents.Thereisafinitequantityofproteinthatmaybecoatedeffectivelyontomicrotiterplates. Useofproteinconcentrationsinexcessoftheoptimalrangewillresultinsurfacesaturationandclumpingontheplatesurface. Thisproduceslowerspecificsignals,andhighernonspecificsignals.
Thecoatingbuffercontainsanantimicrobialagenttoretardbacterialgrowthduringtheplatecoatingprocess,allowingtheproceduretobeperformedatroomtemperature.Dependingontheactivityofthecoatedprotein,platesmaybestoredat2°-8°C(orevenatroomtemperatureforhardyproteins)forseveralmonths,orevenyears.
