TypeIVcollagen(CIV)isamajorstructuralcomponentofthebasallaminathatassemblesasasheetlikenetworkintheunderlyingextracellularspaceofallepithelialcellsandtubes.Thebasallaminaseparatestheepitheliafromtheunderlyingconnectivetissue.ThereareseveraldifferentisoformsoftypeIVcollagen,butallarecomposedinatriplehelicalstructure.Thedifferentisoformsaresynthesizedfromsixgenes:COL4A1,COL4A2,COL4A3,COL4A4,COL4A5andCOL4A6.Type1VcollagensareconsiderablymoreflexIBLethanthefibrillartypecollagens.MutationsingenesassociatedwithtypeIVcollagenareassociatedwithAlportandGoodpasturesyndrome.
Format:Purified,freeze-dried(0.5ml).Reconstitutewith0.5mldiH2Oandstorealiquotsat-20°C.
Specificity(%at1:15000RIAdilution):
RatCollagen,TypeIV:100%
RatCollagen,TypesI,II,III,V:<0.1%
HumancollagentypeI:<0.1%
RatFibronection:<0.1%
MouseLaminin:<0.1%
How_To_Use:
FortheimmunostainingofextraorintracellularcomponentsinlightmicroscopyandthequantitationofrattypeIVcollageninBIOLOGicalfluidsorculturesupernatants.
Applications
- IFA
- IHC(p)
- (SP)RIA
- ELISA
WorkingDilutions
- IFA(withfluoresceinanti-rabbitIgGconjugate):usedilutedat>1:80onfrozenrattissues(skin,liver...)
- IHC(p): usedilutedat≥1:500onfixed,paraffin-embeddedrattissues(skin,liver....)
- ELISA: usedilutedat≥1:200(OD>500).
