Ub-VME is synthesized by the conjugation of 4-amino-but-2-enoic methyl ester to the C-terminus of UbiquitindeltaG76.  Binding of ubiquitin by deubiquitylases (DUBs), for instance UCH’s and most USPs, positions the reactive vinyl bond next to the sulfhydryl-group of the active site cysteine.  Nucleophilic attack by the sulfhydryl on the vinyl bond produces a stable, covalent thioether bond between Ubiquitin and the deubiquitylase.  Thus Ub-VME is a potent suicide inhibitor of DUBs.


Info

• This inhibitor is useful for labeling DUBs in situ as well as preserving the integrity of polyubiquitin chains on modified proteins for analysis or purification.

• Datasheet