Specifications

Cereblon complex, or CRBN complex, is an E3 Ligase that mediates ubiquitination and proteasomal degradation of target proteins. CRBN may play a role in memory and learning behaviors due to its implied interactions with KCTN1. CRBN is typically used as the required E3 ligase in PROTAC (Proteolysis Targeting Chimera) drug design, protein degradation studies and selectivity profiling. In this multi-subunit ubiquitin ligase, Cereblon acts as a substrate adaptor to brings substrate specificity without inherent enzymatic activity. Cereblon is linked to scaffolding protein Cullin 4 (Cul4a) and its regulator ring box proteins (RBX1) via DNA binding protein 1 (DDB1). The Ligase activity of the complex is determined by Cullin-RBX that catalyzes the transfer of ubiquitin from RBX bound E2 to target substrates.

This Cereblon/DDB1/Cul4A/Rbx1 complex is an active CRBN-DCX E3 ubiquitin ligase.

This complex is demonstrated to be active as E3 ligase in enzymatic ubiquitination assays, for evaluating substrate ubiquitination and PROTAC mediated ubiquitination.  This complex is ideal for studying PROTAC interaction with biophysical and HTRF assays for monitoring ternary complex. This complex has Flag tagged proteins suitable for pulldown assays.

Info

Species	Human
Source	HEK293
Tag	Vaarious, see Datasheet
Molecular Weight	Cereblon: 51 kDa; DDB1: 128 kDa; Cul4A: 88 kDa; Rbx1: 13 kDA
Quantity	10 µg, 50 µg
Concentration	Variable
Formulation	40 mm Tris-HCL, pH 8.0, 110 mM NaCl, 2.2 mM KCl, .04% Tween-20 and 20% glycerol
Storage	-80°C, avoid freeze/thaw cycles

Life Sensors的泛素和泛素链泛素是一种小的多肽，通过其C端与目标蛋白上的赖氨酸的ε-氨基缀合。这种缀合称为单泛素化。随后，可以利用遍在蛋白表面上的七个赖氨酸残基（K6，K11，K27，K29，K33，K48，K63）中的任何一个，将额外的遍在蛋白部分与初始遍在蛋白结合。这种泛素链形成称为多泛素化。泛素的酶结合是通过一系列酶进行的，即泛素激活酶E1，泛素结合酶E2和泛素连接酶E3。蛋白质的泛素化在细胞中是可逆的。单泛素化和多泛素化的链均被去泛素化酶催化的水解作用裂解 （DUB）。LifeSensors在遍在泛素途径的所有水平的酶（包括E1，E2和E3酶和DUB）上都有丰富的经验。LifeSensors对蛋白质纯化的广泛了解使我们能够生产大约30种E2酶，20种E3酶和35个DUB，并具有定制表达的能力。此外，我们广泛的检测方法简化了确认酶活性和确定E2-E3对兼容性的过程，并确保您可以选择最能解决希望探索的特定，关键生物学问题的酶。LifeSensors已为您的研究开发了多种泛素衍生物，包括用于结合相互作用研究的DUB耐药性聚泛素链，用于DUB分析和筛选的泛素结合物（荧光团和报告酶），选择性泛素链（二，三和四环素）泛素链）用于DUB特异性和结合测定，位点特异性赖氨酸泛素突变体用于E3连接酶和DUB选择性测定。