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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
| Description |
|---|
| AmajorlimitationofPolymeraseChainReaction(PCR)-basedresearchanddiagnosticandforensictestsistheinhibitionofDNApolymerasesbymanysubstanceslikeasanticoagulants(e.g.,heparin,EDTA,sodiumcitrate)andhemegroup,proteins,polysaccharides,celldebris,andchargedinorganiccompounds,etc.Therefore,DNAisusuallyextractedfromspecimenaccordingtophenol-chloroformmethodorbyanalternative‘silica-membranefilter’method.However,thisistimeconsumingandlaborintensiveandthereisalsoariskofintroducingcontaminationduringthepreparation. EzWayDirectFPCRallowsdirectPCRfrombuccalorbloodFTAdisc,bloodorhairlysatewithoutanyDNApurificationstepsjustbyimmersingtheFTApunchorpipettinglysateintoPCRmixture.EzWayDirectFBuffercontainstheproperamountsofbuffer,MgCl2,dNTPsandthecomponentsthatfacilitatedirectPCRamplification,buthotstartenzymeissuppliedwithseparatetubefortheextendedactivity |
| Specification |
| •DirectPCRfrombuccalorbloodFTAdisc,bloodorhairlysatewithoutanyDNApurificationstepsjustbyimmersingtheFTApunchorpipettinglysateintoPCRmixture |
| Components |
| •EzWayDirectFBufferMix100T(25uLPCR) •HotTaq-fx(5units/uL) •25xInhibitorcocktail |
| Platform |
| •AppliedBiosystems310GeneticAnalyzerfilledwithPOP-4&36cmcapillaries •ABIPRISM®3100/3100-AvantGeneticAnalyzerfilledwithPOP-4&36cmcapillaries •AppliedBiosystems3130/3130xlGeneticAnalyzerfilledwithPOP-4&36cmcapillaries •GeneAmp®PCRSystem9600/9700/Veriti |
| Applications |
| Humanidentificationwithaforensicsample |
| Citation |
