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Koma BioTech/EzWay Genomic DNA Kit, Tissue/K11747/100T

价格
面议
货号:K11747
浏览量:115
品牌:Koma BioTech
服务
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商品描述
Description
EzWay™GenomicDNAKit(Tissue)isanexcellenttoolofferingaspeedyandeconomicmethodtopurifytotalDNAfromtissue.ThistechnologyfirstlysescellsanddegradesproteinbyusingachaotropicsaltandProteinaseK,thenbindsDNAtosilica-basedmembranes,washesDNAwithethanol-containedWashBufferandthenelutespurifiedDNAbylowsaltElutionBufferorddH2O.Comparewithotherharmfulandtime-consumingprocedures,suchasphenol/chloroformextractionandethanolprecipitation,Afterusingthiskit,thehighqualitytotalDNAcanbeuseddirectlyforthedownstreamapplications.
Specification
•BindingCapacity:45ug
•ColumnCapacity:800ul
•RecoveryRate:85-95%
Components
•LysisBuffer
•BindingBuffer
•WashBufferA
•WashBufferB
•ElutionBuffer
•ProteinaseK
•DNA-prepColumn
•2mlCollectionTube
•ElutionTube
Principle
GenomicDNAreleasedfromtissuetreatedbylysisbufferisboundtoasilicagelmembraneinthepresenceofhighconcentrationofchaotropicsalt.ThepurifiedgenomicDNAonthemembraneiselutedintheelutionbuffer.
Applications
•PCRamplifications
•Southernblotting
•Restrictionenzymedigestion
Citation
EzWay Cytokine ELISA Kit通过将样品和检测抗体的孵育整合到1个步骤中,将您的分析时间缩短至2.5小时。另外,它需要的样品量较少(50ul /孔),而通常使用100ul。