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联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description |
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EzWay™GelExtractionKitisdesignedforrapidpurificationofDNAfromstandardagarosegels.Itprovidesafastandsimplemethodusingadvancedsilica-basedmembranetechnologyforrapidandefficientpurificationof100bp-10kbDNAfragments.DNAadsorbstothesilica-membraneinthepresenceofhighsaltwhilecontaminantspassthroughthecolumn.Impuritiesareefficientlywashedaway,andthepureDNAiselutedwithElutionBufferorwater.ThepurifiedDNAissuitableforavarietyofapplicationssuchasfluorescentDNAsequencing,transformation,restrictionmapping,cloning,andlabeling. |
Specification |
•SampleAmount:upto200mgofagarosegel •Recovery:70%-85% •Operationtime:within20min •Elutionvolume:40ul |
Components |
•DNA-prepColumn •CollectionTube •ElutionTube •ExtractionBuffer •WashBuffer •ElutionBuffer |
Principle |
AfteradditionofExtractionBuffertoagarosegelslicesampleandheatingat50-55°Ctomeltgel,DNAisboundtoasilicagelmembraneinthepresenceofhighconcentrationofchaotropicsalt.ThepurifiedDNAproductonthemembraneiselutedinelutionbuffer. |
Applications |
•FluorescentDNAsequencing •Ligation •Transformation •PCR •Micro-injection •DNAmicroarry-basedanalysis •Restrictionmapping •Labelingetc. |
Citation |
■MolecularandPhysiologicalMechanismsofHeavyMetalToleranceinAtriplexhalimus REl-Bakatoushi,AMAlframawy,ATammam InternationalJournalofPhytoremediation2015 ■FurtherMolecularCharacterizationandEffectonHostPhotosyntheticPigmentsandCarbohydratePoolsofanEgyptianIsolateofTBSV FAFattouh,ASAli,RMFathy JPlantPatholMicrob,2015 ■MolecularphylogenyevidenceofaltitudinaldistributionandhABItatadaptationinKoreanEphemeraspecies(Ephemeroptera:Ephemeridae) JMHwang,TJYoon,KISuh,YJBae EntomologicalResearch,2013 ■Identificationandcharacterizationofup-regulatedgenesinthehalophyteLimoniastrummonopetalum(L.)Boissgrownundercrudeoilpollution REl-Bakatoushi JournalofGeneticEngineeringandBiotechnology,2011 |