品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
| Description |
|---|
| EzWay™PCRClean-UpKitisdesignedforrapidpurificationofDNAandremovalofcontaminants.Itprovidesafastandsimplemethodusingadvancedsilica-basedmembranetechnologyforrapidandefficientrecoveryofDNAfragmentsfreeofcontaminantssuchasoligonucleotides,nucleotides,andpolymeraseinyieldsexceedingupto95%.Thesilica-basedmembraneisuniquelyadaptedtoisolateDNAfromaqueoussolutions.ThepurifiedDNAissuitableforavarietyofapplicationssuchasrestrictionenzymedigestion,labeling,ligation,transformation,andsequencingreactions. |
| Specification |
| •SampleAmount:upto100ul•PCRproductorenzymaticreactionproduct •Recovery:85%-95% •Operationtime:within15min •Elutionvolume:40ul |
| Components |
| •DNA-prepColumn •2mlCollectionTube •ElutionTube •BindingBuffer •WashBuffer •ElutionBuffer |
| Principle |
| AfteradditionofDNAbindingbuffertoPCRsample,PCRproductsareboundtoasilicagelmembraneinthepresenceofhighconcentrationofchaotropicsalt.ThepurifiedPCRproductonthemembraneiselutedinelutionbuffer. |
| Applications |
| •Sequencing •Ligation •Digestion •Labeling •Transformationetc. |
| Citation |
| ■PrevalenceofMycobacteriumaviumsubsp.paratuberculosisandEscherichiacoliinbloodsamplesfrompatientswithinflammatoryboweldisease NNazareth,FMagro,EMachado,TGRibeiro MedicalMicroBIOLOGyandImmunology2015 |
