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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
| Description |
|---|
| EzWay™TotalRNAIsolationKitisdesignedforextractionoftotalRNAfromanimaltissueandculturedcells.Somespeciallymodifiedprotocolsaredevelopedforothersamples,suchasbacteriaandyeast.Thismethodfirstlysescellsbyusingachaotropicsalt,thenbindsRNAtosilica-basedmembranes,washesRNAwithethanol-containedwashbufferandthenelutespurifiedRNAbyRNase-freeddH2O.Ittakes30minforanentireprocedure,andthepurifiedRNAisreadyforRT-PCR,northernblotting,primerextensionandCDNAlibraryconstruction. |
| Specification |
| •BindingCapacity:45ug •ColumnCapacity:800ul •RecoveryRate:85-95% •Yield:12ul •A260/A280:2 |
| Components |
| •LysisBuffer •WashBufferA •WashBufferB •DEPC-treatedwater •RNA-prepColumn •2mlCollectionTube •ElutionTube |
| Principle |
| RNAreleasedfromtissuebychaotropicsalt-containinglysisbufferisboundtosilica-basedmembranes.ThepurifiedRNAonthemembraneiselutedwithRNase-freeddH2O. |
| Applications |
| •RT-PCR •Northernblotting •Primerextension •cDNAlibraryconstruction |
| Citation |
| ■TheprophylacticeffectofprobioticBacilluspolyfermenticusKU3againstcancercells NKLee,SHSon,EBJeon,GHJung,JYLee,HDPaik JournalofFunctionalFoods,2015 ■ProbioticpotentialofLactobacillusstrainswithanti-allergiceffectsfromkimchiforyogurtstarters NKLee,SYKim,KJHan,SJEom,HDPaik LWT-FoodScienceandTechnology,2014 |
