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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
| Description |
|---|
| EzWay™One-StepRT-PCRUniversalqMasterMixisdesignedforsensitiveandspecificamplificationone-tubereactionfromtotalRNAtranscripts.Andthiskitisusedthe5’-nucleaseprocessforthequantitativeRT-PCR,suchasTaqManassay. EzWay™One-StepRT-PCRUniversalqMasterMixisapremixedbuffersolutioncontainingMMLVReverseTranscriptaseandHotTaqDNAPolymerase,dNTPsandstABIlizers.ThesolutioncontainstheproperconcentrationofMgCl2anddNTPsforthebetterspecificityandsensitivityandsuppliedas2Xconcentratedtypeforconvenience. SimplyaddprimersandRNAtemplatedilutedinDEPC-treatedwaterintoequalvolumeofEzWay™One-StepRT-PCRUniversalqMasterMix. Pipettingstepsareminimized,reducingthepossibilityoferrorsandcontamination. ThissolutiondoesnotcontainROXreferencedye,whichallowsfluorescencenormalizationoncyclers,suchasABI7000,ABI7300,ABI7700,ABI7900HT,ABI7500,StratageneMx3000,Mx3005P,andMX4000. |
| Specification |
| •SynthesisofCDNAandsequentiallyPCRamplificationfromtargetgeneinone-tube •Real-timequantitativePCRofcDNAtargetsusingfluorescentprobe. •SpecializedbuffercompositionforhighlysensitiveandspecificRNAamplification •Simplesetupandminimaloptimization |
| Components |
| •2XEzWay™One-StepRT-PCRUniversalqMasterMix •4XMagicBuffer |
| Citation |
| ■EvolutionofanavianH5N1influenzaAvirusescapemutant KamelMAHassanin,AhmedSABDel-Moneim WorldJVirol2013November12;2(4):160-169 ■IsolationandmutationtrendanalysisofinfluenzaAvirussubtypeH9N2inEgypt ASAbdel-Moneim,MAAfifi,MFEl-Kady VirologyJournal,2012 |
