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蚂蚁淘在线 / 品牌中心 / Koma BioTech / Koma BioTech/Taq DNA Polymerase Kit II (MgCl2 Free Reaction Buffer)/K0561004/250U
商品描述
Description |
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TaqDNAPolymeraseistheenzymefromtheclonedThermusaquaticusexpressedinE.coli.ThisunmodifiedenzymereplicatesDNAat74°Candexhibitsahalf-lifeof40minutesat95°C.TheenzymecatalyzesthepolymerizationofnucleotidesintoduplexDNAinthe5"→3"directioninthepresenceofmagnesiumandalsopossessesa5"→3"exonucleaseactivity. TaqDNAPolymeraseisrecommendedforuseinPCRbutisnotrecommendedforuseinDNAsequencingreactions. |
Specification |
•Ultrapure,Thermostablerecombinantenzyme •Highlythermostable:10minat97°C,60minat94°C •5"→3"exonucleaseactivity •ExtraAadditionactivity •Amplificationrange: -LamBDaDNA:upto6Kb(Max.12Kb) -HumangenomicDNA:upto2Kb(Max.4Kb) •CompositionofTaqpolymeraseandTaqPCRbufferwithoutMgCl2 •NoNuclease,RNase,Proteasecontamination |
Components |
•TaqDNAPolymeraseII,250U •10XTaqPCRBufferII(MgCl2free) •MgCl2Stocksolution •dNTPMix(2.5mMeach) •10XLoADIngBuffer •NucleaseFreeWater •4XMagicBuffer |
Citation |