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ImmunoStar/Vesicular Monoamine Transporter 1 (VMAT) Rabbit Antibody/20041/1 Ea

价格
¥3276.00
货号:20041
浏览量:127
品牌:ImmunoStar
服务
全国联保
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商品描述

Description

TheImmunoStarVMAT1antiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethodsinratadrenalmedullausingbiotin/avidin-HRPtechniques;theantiserumshowsnoreactivityinratCNS.

Specificityoftheantiserumwasdemonstratedbysolublepre-adsorptionandWesternblot.TissuestainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywithanexcessofratVMAT1peptideresidues502-521. 

Westernblotanalysisofimmunoprecipitatedratadrenalhomogenatesdemonstratesadenseimmunoreactivebandofapproximately55kDandaminorbandofapproximately75kD.

PhotoDescription:IHCimageofVMAT1stainingintheratadrenalmedulla.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforfrozensectioning.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen. 

Host: Rabbit

Quantity/Volume: 100µL

State:LyophilizedWholeSerum

SpeciesReactivity:Rat

AvailABIlity:InStock

AlternateNames: Chromaffingranuleaminetransporter;Solutecarrierfamily18member1;VAT1;VMAT-1;Vesicularaminetransporter1;Slc18a1.Cgat,Vmat1,Vmat-1;solutecarrierfamily18(vesicularmonoamine),member1.Slc18a1,25693,anti-VMAT1

GeneSymbol/ID,Accession#: Slc18a1,25693

RRID: AB_10013883

ImmunoStar的5-HT(血清素)2C受体抗体价格: $ 375.00产品编号:24505使用标准的免疫组织化学方法对ImmunoStar 5-HT2C受体抗血清进行了质量控制测试。使用间接免疫荧光和生物素/抗生物素蛋白-HRP技术,抗血清显示出大鼠脉络丛和海马的强阳性标记。在PBS-Bn / Av-HRP技术中,建议的主要稀释度为1/500-1/1000。镍之类的强化方法将使稀释倍数达到建议的约两倍。通过免疫组织化学和蛋白质印迹对抗体进行表征。蛋白质印迹显示约70 kD的单条带。用过量的合成肽对抗体进行预温育可阻止染色。大鼠脑的免疫组织化学染色与Northern分析,原位杂交和受体放射自显影非常相关。BlastP数据库序列同源性搜索证实,该序列对于大鼠,小鼠和人5-HT2C受体而言是唯一的。图片说明:冠状大鼠脑切片对5-HT2C受体染色的灰度IHC图像(上)和在大鼠大脑皮层中受体表达的高倍放大图像(下)。在取出组织并准备进行切片后,用0.1 M磷酸盐缓冲液中的4%甲醛/0.05%戊二醛固定组织。在进行标准IHC程序之前,将漂浮的切片在室温下于PBS中的10%山羊血清中孵育。一抗以1:1000孵育48小时,随后用PBS洗涤后添加山羊抗兔二抗1小时。用标准生物素-链霉亲和素/ HRP方法和DAB色原进行光学显微镜染色。主持人:兔子数量/体积:100微升状态:液体与反应:小鼠,大鼠别名:   5-hydroxytryptamine receptor 2C;5-HT 2 C;5HT-1C;5-HTR2C;5-羟色胺(5-羟色胺)受体2C,G蛋白偶联,抗5-HT 2CRRID: AB_572212免疫原:大鼠5-HT2C受体(439-460)基因符号  Htr2c数据库链接:  Entrez基因:15560  小鼠  Entrez基因:25187  大鼠