Description
TheImmunoStarVMAT1antiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethodsinratadrenalmedullausingbiotin/avidin-HRPtechniques;theantiserumshowsnoreactivityinratCNS.
Specificityoftheantiserumwasdemonstratedbysolublepre-adsorptionandWesternblot.TissuestainingiscompletelyeliminatedbypretreatmentofthedilutedantibodywithanexcessofratVMAT1peptideresidues502-521.
Westernblotanalysisofimmunoprecipitatedratadrenalhomogenatesdemonstratesadenseimmunoreactivebandofapproximately55kDandaminorbandofapproximately75kD.
PhotoDescription:IHCimageofVMAT1stainingintheratadrenalmedulla.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforfrozensectioning.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
SpeciesReactivity:Rat
AvailABIlity:InStock
AlternateNames: Chromaffingranuleaminetransporter;Solutecarrierfamily18member1;VAT1;VMAT-1;Vesicularaminetransporter1;Slc18a1.Cgat,Vmat1,Vmat-1;solutecarrierfamily18(vesicularmonoamine),member1.Slc18a1,25693,anti-VMAT1
GeneSymbol/ID,Accession#: Slc18a1,25693
RRID: AB_10013883