TheantibodyproducesstronglabelingofVAChTatadilutionof1/3,000-1/5,000usingbiotin-streptavidin/HRPtechniqueinratbasalforebrain.Optimaldilutionwillvarydependinguponfixation,labelingtechniqueand/ordetectionsystem;therefore,adilutionseriesisrecommended.

Immunolabelingiscompletelyabolishedbypre-adsorptionwithsyntheticratVAChT(511-530).Immunolabelingoftransfectedcellsdemonstratesnocrossreactivitywithvesicularmonoaminetransporters.

PhotoDescription:IHCimageofneuronsintheratbasalforebrainstainingforVAT.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%rabbitseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,rabbitanti-goatsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen. 

Host: Goat

Quantity/Volume: 100µL

State:LyophilizedWholeSerum

SpeciesReactivity:Human,Mouse,Rat

AvailABIlity:InStock

AlternateNames: Solutecarrierfamily18,member3;solutecarrierfamily18(vesicularmonoamine)member3;solutecarrierfamily18(vesicularacetylcholine),member3;rVAT;VACht,anti-VAT,anti-VACht 

GeneSymbol/ID,Accession#: Slc18a3,60422

RRID: AB_572269