Description
TheImmunoStarmonoclonalTyrosineHydroxylaseantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratcatecholamineneuronsystemsusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/4000-1/8000withthebiotin/avidin-HRPtechnique. ThisantibodyhasbeenusedsuccessfullyforIHC,ICC,FC,andIP.
WesternBlot: Thisantibodydoesnotcrossreactwithdihydropterdinereductase,dopamine-B-hydroxylase,phenylethanolamine-N-methyltransferase,phenylalaninehydroxylaseortryptophanhydroxylaseusingWesternBlotmethods.ClickhereformoreWesternBlotprotocolinformation.
TheTHantibodyidentifiesdopaminergicaxonsandnerveendingduringearlymouse,ratandhumandevelopment.Thisisbecausetyrosinehydroxylaseisexpressedveryearlyinthedevelopingdopamineneuronphenotype.
PhotoDescription:Highmagnification(above)andlowmagnification(below)IHCimageoftheratmidbrainstainingfortyrosinehydroxylase.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%rabbitseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,rabbitanti-mousesecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host:Mouse
Quantity/Volume:100µL
State:LyophilizedWholeSerum
ReactsWith:Alpaca(Llama),Amphibian,Anuran(Frog),Anuran(Urodele),AplysiaCalifornica(SeaSlug),Bee,Bird,Blowfly,Boar,Budgerigars,Canary,Cat,Chick,Chicken,Clam,Cockroach,Cod(Fish),Crab,Crayfish,Dog,Drosophila(Fly),Eel,Emu,EnegalBichirs(Eel),Ewe,Ferret,Fish,Fly,Fox,Frog(XenopusLaevis),FruitFly,Gastropod(Slug),Gastropoda,Gecko,Gerbil,Goldfish,GuineaPig,Guppies,Hamster(MesocricetusAuratus),HelisomaDuryi(Snail),Hen,Human,Iguana,IlyanassaObsoleta(SeaSnail),Lamprey,Leech,Lizard,Lobster,Locust,MexicanAxolotl(Salamander),Mollusca,Monkey(MacacaFascicularis),Mouse,Newt,Opossum,Parrot,PeriplanetaAmericana(Cockroach),PhestillaSibogae(SeaCoral),PhestillaSibogae(SeaSlug),PhoronisPallida(Phoronida),Pig,Pigeon,Possum,Prawn,Quail,Rabbit,Rat,RenillaKoellikeri(SeaPansy),Rodent,Salamander,Salmon,SeaFish,SeaSlug,Shark,Sheep,Short-BeakedEchidna,Shrew,Snail,Snake,Sparrow,Spisula(Clam),SquirrelMonkey,Starling,Stickleback,Tadpole,Trout,Turkey,Turtle,UrodeleAmphibian,WaterBuffalo,Worm,Yeast,ZebraFinch,Zebrafish
AvailABIlity:InStock
AlternateNames: Tyrosine3-monooxygenase(TH);TYH;Tyrosine3-hydroxylase,anti-TH,anti-tyrosinehydroxylase
GeneSymbol/ID,Accession#: Th,25085