Description
TheImmunoStarSubstancePantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratsubstantianigraandspinalcordusingbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/6000-1/8000inPBS/0.3%TritonX-100-biotin/avidin-HRPTechnique.
ThespecificityoftheantiserumforSubstancePwasdemonstratedusingsolublepre-adsorptionwiththepeptidesinquestionatafinalconcentrationof10µgofpeptidepermLofdilutedantiserum.SubstancePimmunolabelingwascompletelyabolishedbypre-adsorptionwithSubstanceP.Pre-adsorptionwiththefollowingpeptidesresultedinnoreductionofimmunostaining:neurokininA,neurokininB,somatostatinandneuropeptideK.
PhotoDescription:IHCimageofratspinalcord(above)andrattegmentum(below)stainingforsubstancep.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioningforbrainandfrozensectioningforspinalcord.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:Bird,Bullfrog,Cat,Chick,Chicken,Dog,Dolphin,Doves,Equine,Fish,Frog,Gerbil,Goat,Goldfish,GuineaPig,Hamster,Horse,Human,Monkey,Mouse,Mudpuppy,Narwhal,Parrot,Pig,Pig,Pigeon,Quail,Rabbit,Ram(Sheep),RanaPipiens(LeopardFrog),Rat,Ratfish,Salmon,ScorpaenaPorcus(Fish),SeaLamprey,Sheep,Snail,SparusAurata(Fish),SqualusAcanthias(Dogfish),Squirrel,Starfish,StingRay,Sturgeon(Fish),Three-SpinedStickleback(Fish),Trout,Turtle,Vole,ZebraFinch,Zebrafish
AvailABIlity:InStock
AlternateNames: Protachykinin-1;PPT;tachykininpeptidehormone;NKA,NKNA,TAC2;tachykinin,precursor1,anti-substanceP
GeneSymbol/ID,Accession#: TAC1,6863
RRID: AB_572266