Description
TheantibodyhasaprovenstrongBiotin-Streptavidin/HRPstainingata1/1000-1/2000dilutioninrathypothalamus(medianeminence).Thespecificityoftheantiserumwasexaminedbysolublepre-adsorptionwiththepeptidesinquestionatafinalconcentrationof106M.
Somatostatinimmunolabelingwascompletelyabolishedbypre-adsorptionwithsomatostatin,somatostatin25,andsomatostatin28.Pre-adsorptionwiththefollowingpeptidesresultedinnoreductionofimmunostaining:substanceP,amylin,glucagon,insulin,neuropeptideY,andVIP.
PhotoDescription:IHCimageofthemedianeminencestainingforsomatostatin(above).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.Florescentsecondary(below).
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:Alligator,Anuran(Frog),Bat,Bird,Buffalo(BubalusBubalis),Bullfrog,Cat,Chick,Chicken,DermophisMexicanusMexicanCaecilian (Amphibian),Dog,Duck,Fish,Fowl,Frog(XenopusLaevis),GuineaPig,Hamster,Human,Lizard,MalayanPangolin(SundaPangolin),ManductaSexta(Moth),Monkey,Mouse,Pig,Pigeon,Quail,Rabbit,Raccoon,RanaPipiens(LeopardFrog),Rat,ScorpaenaPorcus(Fish),SeaBass(Fish),Sheep,Snail,Snake,SparusAurata(Fish),Starfish,StingRay,Turtle,Zebrafish
AvailABIlity:InStock
PreabsorptionControl:AvailableatSigmaAldrich,Catalog#S9129
AlternateNames: Growthhormonerelease-inhibitingfactor;Somatostatin14;Somatostatin28;prepro-somatostatin;SMST;anti-somatostatin
GeneSymbol/ID,Accession#: SST,6750
RRID: AB_572264