Description
TheImmunoStarOxytocinantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofrathypothalamususingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/4,000-1/8,000inPBS/0.3%TritonX-100-Bn/Av-HRP.
Stainingiscompletelyeliminatedbypretreatmentof1mLofthedilutedantibodywith5µgofOxytocin.Pretreatmentof1mLofthedilutedantibodywithasmuchas100µgofvasopressindoesnotdiminishstaining.
PhotoDescription: IHCimageofneuronsstainingforoxytocinintherathypothalamus(above)andhighermagnificationoftherathypothalamuswithnickelpreparation(below).Thetissuewasfixedwith4%formaldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:Bat,Bovine,Chicken,Dog,Frog(XenopusLaevis),GuineaPig,Hamster,Human,ManductaSexta(Moth),MelopsittacusUndulatus(Bird),MoleRat,Monkey,Mouse,Rat,Sheep,Vole
AvailABIlity:InStock
AlternateNames: Oxytocin-neurophysin1;Ocytocin;neurophysinI;oxytocin,prepropeptide;oxytocin,prepro-(neurophysinI);oxytocin-neurophysinI,preproprotein;OT;OT-NPI;OXT-NPI;oxytocin/neurophysinIprepropeptide,anti-Oxytocin
GeneSymbol/ID,Accession#: OXT,5020
RRID: AB_572258