Description
TheImmunoStarNeuropeptideYY1Receptorwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratcortex,arcuateandhippocampususingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionsare1/500-1/1000inPBS-Bn/Av-HRPdetection.
TheantibodywascharacterizedbyimmunohistochemistryandWesternblot.Westernblotshowedoneimmunoreactivebandof40kDandasinglehighmolecularweightband,presumablyaprecursormolecule.
Preincubationoftheantibodywithanexcessofthesyntheticpeptideblockedstaining.ImmunohistochemicalstainingofratbraincorrelateswellwithNorthernanalysis,insituhybridizationandreceptorautorADIography.BlastPdatabasesequencehomologysearchesconfirmedthatthissequenceisuniquetorat,mouseandhumanNPYY1receptors.
PhotoDescription:IHCimageoftheratcortexstainingfortheneuropeptideYY1recptor.Thetissuewasfixedwith4%formaldehyde/0.05%glutaraldehydein0.1Mphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State: Liquid
ReactsWith:Hamster,Human,Mouse,Rat
AvailABIlity:InStock
AlternateNames: NeuropeptideYreceptor1;NPYreceptor1;NPYR;NPYY1;NPY1-R;NeuropeptideYreceptortype1;FC5;NPYR1,neuropeptideY1receptor,anti-NPYY1
GeneSymbol/ID,Accession#: neuropeptideYreceptorY1,anti-NPYY1
RRID: AB_572257