Theantibodyhasaprovenstrongbiotin-streptavidin/HRPstainingat1/1,000-1/1,200dilutioninratglobuspallidusandamygdala.

Stainingiscompletelyeliminatedbypretreatmentwith100µgofmethionineenkephalinpermLofdilutedantiserum.Pretreatmentwith100µgofleucineenkephalinonlypartiallyblocksstaining.

PhotoDescription: Highmagnificationoftheglobuspalliduswithnickelpreparation(above)andlowmagnificationIHCimageofmethionineencephalinstainingintheglobuspallidusandamygdalafortheratbrain(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:2000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.

Host: Rabbit

Quantity/Volume: 100µL

State:LyophilizedWholeSerum

ReactsWith:AstyanaxJordani(Fish),Bird,Blowfly,Bovine,Bullfrog,Cat,Chick,Chicken,Cockroach,Cod,Crab,Dog,Fish,Frog,Gerbil,Goldfish,GuineaPig,Hamster,Human,Lamprey,Leech,LymnaeaStagnalis(Snail),ManductaSexta(Moth),Monkey,Mouse,Octopus,Parrot,Pig,Pigeon,Rabbit,RanaEsculenta(Frog),RanaPipiens(LeopardFrog),RanaTemporaria(Frog),Rat,Ratfish,Salamander,Salmon,SeaBass(Fish),Shrew,SquirrelMonkey,Starling,Tapeworm,Turtle,ZebraFinch

AvailABIlity:InStock

AlternateNames: Enkephalin(Met);Proenkephalin-A;Met-enkephalin;Opioidgrowthfactor;OGF,anti-MethionineEnkephalin,MetEnk

GeneSymbol/ID,Accession#: PENK,5179

RRID: AB_572250