Description
TheImmunoStarInsulinantiserumwasqualitycontroltestedusingstandardimmunohistochemicalmethods.Theantiserumdemonstratesstronglypositivelabelingofratpancreaticbetacellsusingindirectimmunofluorescentandbiotin/avidin-HRPtechniques.Recommendedprimarydilutionis1/3000-1/5000inPBS/0.3%TritonX-100-biotin/avidin-HRPTechnique.
Immuostainingiscompletelyabolishedbysolublepre-adsorptionwithbovineinsulinat10µgpermLofdilutedantiserum.
PhotoDescription:IHCimageofbetacellsintheisletsofLangerhansoftheratpancreasstainingforinsulin(above)andcounterstainedwithhemotoxylin(below).Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforfrozensectioning.SectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen.
Host: Rabbit
Quantity/Volume: 100µL
State:LyophilizedWholeSerum
ReactsWith:BubalusBubalis(Buffalo),Cattle,Chick,Cow,Heifer(Cow),Horse,Human,Lizard,Macaque,ManductaSexta(Moth),Monkey,Mouse,Pig,Rat,Sheep
AvailABIlity:InStock
AlternateNames: anti-Insulin
GeneSymbol/ID,Accession#: INS,397415,280829
RRID: AB_572246